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murine alveolar macrophage cell line  (ATCC)
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GBD inhibits LTA-induced <t>macrophage</t> migration through Nrf2 activation. MH-S cells were pretreated with the indicated compounds for 30 min and then stimulated with LTA. (A) Representative wound healing images 0, 6, and 24 h after LTA stimulation. (B) Quantification of wound closure. (C) Representative images of Transwell migration at 6 h and 24 h after LTA stimulation. Migrated cells on the lower surface of the membrane were fixed, stained with crystal violet and images captured under an inverted light microscope. Scale bar, 200 µm. (D) Quantification of migrated cells. Data are presented as means ± SD. ***P<0.001 vs. DMSO; ### P<0.001 vs. DMSO+LTA; † P<0.05, †† P<0.01 and ††† P<0.001 vs. GBD+LTA; ‡‡ P<0.01 vs. ML385+LTA. GBD, glabridin; LTA, lipoteichoic acid; Nrf2, nuclear factor erythroid 2-related factor 2; DMSO, dimethyl sulfoxide.
Murine Alveolar Macrophage Cell Line, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/murine alveolar macrophage cell line/product/ATCC
Average 99 stars, based on 1 article reviews
murine alveolar macrophage cell line - by Bioz Stars, 2026-03
99/100 stars
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GBD inhibits LTA-induced macrophage migration through Nrf2 activation. MH-S cells were pretreated with the indicated compounds for 30 min and then stimulated with LTA. (A) Representative wound healing images 0, 6, and 24 h after LTA stimulation. (B) Quantification of wound closure. (C) Representative images of Transwell migration at 6 h and 24 h after LTA stimulation. Migrated cells on the lower surface of the membrane were fixed, stained with crystal violet and images captured under an inverted light microscope. Scale bar, 200 µm. (D) Quantification of migrated cells. Data are presented as means ± SD. ***P<0.001 vs. DMSO; ### P<0.001 vs. DMSO+LTA; † P<0.05, †† P<0.01 and ††† P<0.001 vs. GBD+LTA; ‡‡ P<0.01 vs. ML385+LTA. GBD, glabridin; LTA, lipoteichoic acid; Nrf2, nuclear factor erythroid 2-related factor 2; DMSO, dimethyl sulfoxide.

Journal: Molecular Medicine Reports

Article Title: Glabridin attenuates LTA-induced alveolar macrophage migration via activation of the Nrf2/HO-1 pathway

doi: 10.3892/mmr.2025.13758

Figure Lengend Snippet: GBD inhibits LTA-induced macrophage migration through Nrf2 activation. MH-S cells were pretreated with the indicated compounds for 30 min and then stimulated with LTA. (A) Representative wound healing images 0, 6, and 24 h after LTA stimulation. (B) Quantification of wound closure. (C) Representative images of Transwell migration at 6 h and 24 h after LTA stimulation. Migrated cells on the lower surface of the membrane were fixed, stained with crystal violet and images captured under an inverted light microscope. Scale bar, 200 µm. (D) Quantification of migrated cells. Data are presented as means ± SD. ***P<0.001 vs. DMSO; ### P<0.001 vs. DMSO+LTA; † P<0.05, †† P<0.01 and ††† P<0.001 vs. GBD+LTA; ‡‡ P<0.01 vs. ML385+LTA. GBD, glabridin; LTA, lipoteichoic acid; Nrf2, nuclear factor erythroid 2-related factor 2; DMSO, dimethyl sulfoxide.

Article Snippet: MH-S cells, a murine alveolar macrophage cell line, were obtained from ATCC (cat. no. CRL-2019) and cultured in RPMI-1640 medium (cat. no. A1049101; Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS; cat. no. 26140079; Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin.

Techniques: Migration, Activation Assay, Membrane, Staining, Light Microscopy

Schematic of GBD-induced modulation of macrophage migration in vitro . Upon LTA stimulation, MH-S cells increase ROS production, activating Nrf2 and upregulating HO-1. GBD thus modulateS cell migration by increasing Nrf2 nuclear translocation and HO-1 expression. GBD, glabridin; LTA, lipoteichoic acid; ROS, reactive oxygen species; Nrf2, nuclear factor erythroid 2-related factor 2; HO-1, heme oxygenase-1.

Journal: Molecular Medicine Reports

Article Title: Glabridin attenuates LTA-induced alveolar macrophage migration via activation of the Nrf2/HO-1 pathway

doi: 10.3892/mmr.2025.13758

Figure Lengend Snippet: Schematic of GBD-induced modulation of macrophage migration in vitro . Upon LTA stimulation, MH-S cells increase ROS production, activating Nrf2 and upregulating HO-1. GBD thus modulateS cell migration by increasing Nrf2 nuclear translocation and HO-1 expression. GBD, glabridin; LTA, lipoteichoic acid; ROS, reactive oxygen species; Nrf2, nuclear factor erythroid 2-related factor 2; HO-1, heme oxygenase-1.

Article Snippet: MH-S cells, a murine alveolar macrophage cell line, were obtained from ATCC (cat. no. CRL-2019) and cultured in RPMI-1640 medium (cat. no. A1049101; Gibco; Thermo Fisher Scientific, Inc.) supplemented with 10% fetal bovine serum (FBS; cat. no. 26140079; Gibco; Thermo Fisher Scientific, Inc.) and 1% penicillin-streptomycin.

Techniques: Migration, In Vitro, Translocation Assay, Expressing